[Microbiology Question] How do you determine motility of an endospore?
Q. When observe an endospore under a microscope and find it to be large and swollen, almost like a big round pocket, but after a few weeks, you repeat the same process of staining and mounting on a glass and re-observing it under a microscope, but then you find that the old large round spore cell is gone only to discover a hundred different round spore cells under a microscope. Would this endospore microbacterium be considered positive for motility?
Asked by Ice_Queen - Fri May 25 18:17:04 2007 - - 1 Answers - 0 Comments
A. No. Spores are not motile, only the Firmicute bacteria themselves are. The simplest way to determine motility is to either observe them under a microscope or plate them out in a soft agar plate (less agar than the normal plates). Motile bacteria will spread out within the agar into a diffuse, large colony while non-motile ones will form tight, compact colonies.
Answered by Mark S, JPAA - Fri May 25 18:24:46 2007
Q. When observe an endospore under a microscope and find it to be large and swollen, almost like a big round pocket, but after a few weeks, you repeat the same process of staining and mounting on a glass and re-observing it under a microscope, but then you find that the old large round spore cell is gone only to discover a hundred different round spore cells under a microscope. Would this endospore microbacterium be considered positive for motility?
Asked by Ice_Queen - Fri May 25 18:17:04 2007 - - 1 Answers - 0 Comments
A. No. Spores are not motile, only the Firmicute bacteria themselves are. The simplest way to determine motility is to either observe them under a microscope or plate them out in a soft agar plate (less agar than the normal plates). Motile bacteria will spread out within the agar into a diffuse, large colony while non-motile ones will form tight, compact colonies.
Answered by Mark S, JPAA - Fri May 25 18:24:46 2007
What does it mean when you are asked for the Endospore position?
Q. I'm in a Micro Biology class and for our Lab homework we are suppose write the Endospore position. Do you know what that means? and can you give me an example. Thanks!
Asked by cynthia s - Wed Feb 3 12:12:06 2010 - - 1 Answers - 0 Comments
A. When you look at Bacillus spp under a microscope endospores may be seen within the cells If the spore in in the middle of the rod shaped cell it is "Central" If it is right at one end it is "Terminal" If it is about half way between the middle and end it is "Sub-terminal" If the spore is itself rod-shaped and at an angle to the cell so that it sticks out it is "Lateral" In any one species of Bacillus all the spores behave the same way and so this characteristic can help tell one species from another
Answered by Ski rkae - Wed Feb 3 13:27:46 2010
Q. I'm in a Micro Biology class and for our Lab homework we are suppose write the Endospore position. Do you know what that means? and can you give me an example. Thanks!
Asked by cynthia s - Wed Feb 3 12:12:06 2010 - - 1 Answers - 0 Comments
A. When you look at Bacillus spp under a microscope endospores may be seen within the cells If the spore in in the middle of the rod shaped cell it is "Central" If it is right at one end it is "Terminal" If it is about half way between the middle and end it is "Sub-terminal" If the spore is itself rod-shaped and at an angle to the cell so that it sticks out it is "Lateral" In any one species of Bacillus all the spores behave the same way and so this characteristic can help tell one species from another
Answered by Ski rkae - Wed Feb 3 13:27:46 2010
What color do you expect to see if you do a Gram stain on an endospore-producing bacterium?
Q. What color do you expect to see if you do a Gram stain on an endospore-producing bacterium? Choose one answer. a. green b. red c. purple d. blue ok so im not sure but i think i've narrowed it down to purple or red. But im not sure.
Asked by caseychimento - Sun Feb 1 15:56:58 2009 - - 4 Answers - 0 Comments
A. If they are rods (bacillus), then they are gram positive, endospore-forming rods, and the answer would be violet (purple), answer c.
Answered by N E - Sun Feb 1 16:33:30 2009
Q. What color do you expect to see if you do a Gram stain on an endospore-producing bacterium? Choose one answer. a. green b. red c. purple d. blue ok so im not sure but i think i've narrowed it down to purple or red. But im not sure.
Asked by caseychimento - Sun Feb 1 15:56:58 2009 - - 4 Answers - 0 Comments
A. If they are rods (bacillus), then they are gram positive, endospore-forming rods, and the answer would be violet (purple), answer c.
Answered by N E - Sun Feb 1 16:33:30 2009
What impact does endospore formation have on food safety?
Q. What impact does endospore formation have on food safety?
Asked by surf789987 - Thu Feb 19 18:42:21 2009 - - 1 Answers - 0 Comments
A. Endospores are dormant cells that are very resistant to heat. When home-canning, or in foods that are pasteurized, spores may not be killed, and can germinate either in the food or in the body when consumed. Botulism is caused in this way. The bacterial spores germinate in the canned food, and produce botulinum toxin. You eat the food, and get botulism.
Answered by Jay M - Thu Feb 19 19:22:43 2009
Q. What impact does endospore formation have on food safety?
Asked by surf789987 - Thu Feb 19 18:42:21 2009 - - 1 Answers - 0 Comments
A. Endospores are dormant cells that are very resistant to heat. When home-canning, or in foods that are pasteurized, spores may not be killed, and can germinate either in the food or in the body when consumed. Botulism is caused in this way. The bacterial spores germinate in the canned food, and produce botulinum toxin. You eat the food, and get botulism.
Answered by Jay M - Thu Feb 19 19:22:43 2009
What is a bacteria endospore and how is it an advantage for the bacteria?
Q. What is a bacteria endospore and how is it an advantage for the bacteria?
Asked by Kiddy - Tue Apr 10 18:16:51 2007 - - 2 Answers - 0 Comments
A. An endospore is a very thick-shelled structure formed inside a bacterial cell when the conditions are bad for the bacteria. The endospore can withstand the adverse conditions and develop back into the regular bacterial form when conditions are better. Bad conditions for bacteria: not enough water, wrong temp, ... Here are some pictures:
Answered by ecolink - Tue Apr 10 18:21:30 2007
Q. What is a bacteria endospore and how is it an advantage for the bacteria?
Asked by Kiddy - Tue Apr 10 18:16:51 2007 - - 2 Answers - 0 Comments
A. An endospore is a very thick-shelled structure formed inside a bacterial cell when the conditions are bad for the bacteria. The endospore can withstand the adverse conditions and develop back into the regular bacterial form when conditions are better. Bad conditions for bacteria: not enough water, wrong temp, ... Here are some pictures:
Answered by ecolink - Tue Apr 10 18:21:30 2007
What advantage to a bacterium would an endospore be?
Q. What advantage to a bacterium would an endospore be?
Asked by bchbum2007 - Mon Sep 21 18:12:33 2009 - - 1 Answers - 0 Comments
A. The bacterium could survive as an endospore in a dry environment until such time as a moisture film returned so the bacteria could actively live.
Answered by emucompboy - Mon Sep 21 19:21:48 2009
Q. What advantage to a bacterium would an endospore be?
Asked by bchbum2007 - Mon Sep 21 18:12:33 2009 - - 1 Answers - 0 Comments
A. The bacterium could survive as an endospore in a dry environment until such time as a moisture film returned so the bacteria could actively live.
Answered by emucompboy - Mon Sep 21 19:21:48 2009
What is the purpose of the endospore stain?
Q. What is the purpose of the endospore stain?
Asked by R C - Mon Jul 7 19:09:57 2008 - - 2 Answers - 0 Comments
A. I'm assuming that an endospore stain would alert one to the presence of an endospore within the organism which you are staining. An endospore is a structure usually found in bacterium that allow them to survive through severe conditions such as high acidity, low O2 levels and high/low heat. The most dangerous bacteria noted to have endospores are Botulism which can lived in canned food for undefined periods of time.
Answered by Becca J - Mon Jul 7 20:09:06 2008
Q. What is the purpose of the endospore stain?
Asked by R C - Mon Jul 7 19:09:57 2008 - - 2 Answers - 0 Comments
A. I'm assuming that an endospore stain would alert one to the presence of an endospore within the organism which you are staining. An endospore is a structure usually found in bacterium that allow them to survive through severe conditions such as high acidity, low O2 levels and high/low heat. The most dangerous bacteria noted to have endospores are Botulism which can lived in canned food for undefined periods of time.
Answered by Becca J - Mon Jul 7 20:09:06 2008
Does anyone know how Endospore staining works (Microbiology Lab) ?
Q. Does anyone know how the following work: What color (dye) is taken up by the endospores in simple staining? What color (dye) is taken up by the cell wall? How would the endospore appear inside Clostridia that were simple stained? What are some medically important diseases caused by Bacillus and Clostridium? How can endospores be destroyed? Thanks for any help!! I greatly appreciate it!
Asked by Memphis Tiger fan - Sun Sep 27 15:15:57 2009 - - 1 Answers - 0 Comments
Q. Does anyone know how the following work: What color (dye) is taken up by the endospores in simple staining? What color (dye) is taken up by the cell wall? How would the endospore appear inside Clostridia that were simple stained? What are some medically important diseases caused by Bacillus and Clostridium? How can endospores be destroyed? Thanks for any help!! I greatly appreciate it!
Asked by Memphis Tiger fan - Sun Sep 27 15:15:57 2009 - - 1 Answers - 0 Comments
What are the major steps involved in endospore formation?
Q. What are the major steps involved in endospore formation?
Asked by bacteria - Sun Feb 25 00:23:30 2007 - - 1 Answers - 0 Comments
A. Endospores and Endospore Staining --- Endospore Formation Endospores are formed by vegetative cells in a process called sporulation. Sporulation is initiated when conditions for growth of the vegetative cells become harsh (for example, when water or an essential nutrient is limiting). Sporulation is a complex process involving as many as 200 genes. These genes are activated by an environmental trigger, causing sporulation of the cell. The steps leading to endospore formation result in the creation of a dry, metabolically inert and extremely resistant endospore from a moist, metabolically active vegetative cell. Extensive sporulation studies have been done on Bacillus subtilis, and this microbe can perform the entire… [cont.]
Answered by dcny21 - Sun Feb 25 00:28:40 2007
Q. What are the major steps involved in endospore formation?
Asked by bacteria - Sun Feb 25 00:23:30 2007 - - 1 Answers - 0 Comments
A. Endospores and Endospore Staining --- Endospore Formation Endospores are formed by vegetative cells in a process called sporulation. Sporulation is initiated when conditions for growth of the vegetative cells become harsh (for example, when water or an essential nutrient is limiting). Sporulation is a complex process involving as many as 200 genes. These genes are activated by an environmental trigger, causing sporulation of the cell. The steps leading to endospore formation result in the creation of a dry, metabolically inert and extremely resistant endospore from a moist, metabolically active vegetative cell. Extensive sporulation studies have been done on Bacillus subtilis, and this microbe can perform the entire… [cont.]
Answered by dcny21 - Sun Feb 25 00:28:40 2007
Why use steam during an endospore stain in Microbiology?
Q. Why use steam during an endospore stain in Microbiology?
Asked by I'm Awesome - Thu Mar 11 09:57:35 2010 - - 0 Answers - 0 Comments
Q. Why use steam during an endospore stain in Microbiology?
Asked by I'm Awesome - Thu Mar 11 09:57:35 2010 - - 0 Answers - 0 Comments
in the Dorner endospore stain,a smear covered with carbolfuchsin is steamed, then decolorized with acid?
Q. alcohol and counterstained with nigrosin. Describe the microscopic appearance after this procedure.
Asked by apple1985 - Tue Feb 19 15:54:08 2008 - - 1 Answers - 0 Comments
A. If the cell has an endospore, the heating should drive the pink carbolfuchsin stain into it. You would see the endospores and maybe the exterior of some cells, which can be enhanced with a counterstain. This is very similar to an acid fast stain.
Answered by Yersinia pestis - Tue Feb 19 17:21:56 2008
Q. alcohol and counterstained with nigrosin. Describe the microscopic appearance after this procedure.
Asked by apple1985 - Tue Feb 19 15:54:08 2008 - - 1 Answers - 0 Comments
A. If the cell has an endospore, the heating should drive the pink carbolfuchsin stain into it. You would see the endospores and maybe the exterior of some cells, which can be enhanced with a counterstain. This is very similar to an acid fast stain.
Answered by Yersinia pestis - Tue Feb 19 17:21:56 2008
What are the five genera of bacteria that form endospore?
Q. What are the five genera of bacteria that form endospore?
Asked by bacteria - Sun Feb 25 00:08:51 2007 - - 2 Answers - 0 Comments
A. Bacillus, Clostridium, Desulfotomaculum, Sporolactobacillus and Sporosarcina. Good luck with whatever microbiology project this comes from!!
Answered by edie t - Sun Feb 25 00:24:09 2007
Q. What are the five genera of bacteria that form endospore?
Asked by bacteria - Sun Feb 25 00:08:51 2007 - - 2 Answers - 0 Comments
A. Bacillus, Clostridium, Desulfotomaculum, Sporolactobacillus and Sporosarcina. Good luck with whatever microbiology project this comes from!!
Answered by edie t - Sun Feb 25 00:24:09 2007
What is the function of a bacterial endospore?
Q. What is the function of a bacterial endospore?
Asked by adebolaji22 - Mon Oct 30 14:53:47 2006 - - 2 Answers - 0 Comments
A. when essential nutrients are depleted, ceratin gram positive bacteria form these endospores.They are very duable and can with stand extreme heat, cold, lack of water, exposure to mony toxic chemicals and still survive. Thier essential function is to carry on the bacteria's life, they ensure the survival of a bacterium through periods of environmental stress. and can live for millions of years!
Answered by tiff-so-fierce - Mon Oct 30 15:41:55 2006
Q. What is the function of a bacterial endospore?
Asked by adebolaji22 - Mon Oct 30 14:53:47 2006 - - 2 Answers - 0 Comments
A. when essential nutrients are depleted, ceratin gram positive bacteria form these endospores.They are very duable and can with stand extreme heat, cold, lack of water, exposure to mony toxic chemicals and still survive. Thier essential function is to carry on the bacteria's life, they ensure the survival of a bacterium through periods of environmental stress. and can live for millions of years!
Answered by tiff-so-fierce - Mon Oct 30 15:41:55 2006
Do specimens need be in fixed smears in order to perform either gram stain or endospore stain?
Q. Thanks!
Asked by Stephanie and Renae D - Mon May 19 23:50:36 2008 - - 1 Answers - 0 Comments
A. Yes, specimens need to be heat-fixed on the slide so that the material will not fall off during staining.
Answered by OKIM IM - Tue May 20 00:20:03 2008
Q. Thanks!
Asked by Stephanie and Renae D - Mon May 19 23:50:36 2008 - - 1 Answers - 0 Comments
A. Yes, specimens need to be heat-fixed on the slide so that the material will not fall off during staining.
Answered by OKIM IM - Tue May 20 00:20:03 2008
what is the name of bacterium which is a gram positive, rod shaped, endospore-forming microbe?
Q. this bacterium also can be found living in the soil where the pilot works
Asked by zena c - Tue Dec 11 02:11:51 2007 - - 2 Answers - 0 Comments
A. clostridium ... I believe
Answered by loveSol - Tue Dec 11 02:27:03 2007
Q. this bacterium also can be found living in the soil where the pilot works
Asked by zena c - Tue Dec 11 02:11:51 2007 - - 2 Answers - 0 Comments
A. clostridium ... I believe
Answered by loveSol - Tue Dec 11 02:27:03 2007
what is the different between bacterial endospore and capsule?
Q. the differences
Asked by sakura - Mon Oct 19 16:17:39 2009 - - 2 Answers - 0 Comments
A. Bacterial capsule is made up of polysaccharide units or protiens or both.Gives sticky characteristic 2 bacteria.helps in attachment whereas endospore is formed during unfaaavourable conditions and on favourable conditions form a bacterial cell & has stored food.
Answered by twinkle - Mon Oct 19 16:24:15 2009
Q. the differences
Asked by sakura - Mon Oct 19 16:17:39 2009 - - 2 Answers - 0 Comments
A. Bacterial capsule is made up of polysaccharide units or protiens or both.Gives sticky characteristic 2 bacteria.helps in attachment whereas endospore is formed during unfaaavourable conditions and on favourable conditions form a bacterial cell & has stored food.
Answered by twinkle - Mon Oct 19 16:24:15 2009
If endospores can be seen by simple staining, why not use this technique instead of endospore staining?
Q. If endospores can be seen by simple staining, why not use this technique instead of endospore staining?
Asked by sweetie-d - Fri Feb 1 18:26:07 2008 - - 1 Answers - 0 Comments
A. Because when using the Malachite Green endospore stain, it is hard to mistake other things for spores (unless you are dealing with Mycobacteria - they are not spore formers but their mycolic acids can look similar to sporeformers under a microscope when using malachite green). When you are doing the spore stain, you can even see green endospores inside the vegetative cells. That would be pretty hard to pick out with just a simple stain. HTH!
Answered by Doublemint - Fri Feb 1 19:03:19 2008
Q. If endospores can be seen by simple staining, why not use this technique instead of endospore staining?
Asked by sweetie-d - Fri Feb 1 18:26:07 2008 - - 1 Answers - 0 Comments
A. Because when using the Malachite Green endospore stain, it is hard to mistake other things for spores (unless you are dealing with Mycobacteria - they are not spore formers but their mycolic acids can look similar to sporeformers under a microscope when using malachite green). When you are doing the spore stain, you can even see green endospores inside the vegetative cells. That would be pretty hard to pick out with just a simple stain. HTH!
Answered by Doublemint - Fri Feb 1 19:03:19 2008
of what advantage to Clostridium is an endospore?
Q. of what advantage to Clostridium is an endospore?
Asked by Dorya - Wed Feb 18 11:38:33 2009 - - 1 Answers - 0 Comments
A. When conditions in the environment where a Clostridium resides become unfavorable it will form an endospore allowing it to remain in a dorment "hibernation" type state until conditions improve.
Answered by gambitace0913 - Wed Feb 18 11:58:02 2009
Q. of what advantage to Clostridium is an endospore?
Asked by Dorya - Wed Feb 18 11:38:33 2009 - - 1 Answers - 0 Comments
A. When conditions in the environment where a Clostridium resides become unfavorable it will form an endospore allowing it to remain in a dorment "hibernation" type state until conditions improve.
Answered by gambitace0913 - Wed Feb 18 11:58:02 2009
what is endospore formation?
Q. help!! i can't find the definition anywhere on Google in an easy to understand way.
Asked by isabelle is cool - Wed Mar 25 22:27:38 2009 - - 1 Answers - 0 Comments
A. When a bacterium detects environmental conditions are becoming unfavourable it may start the process of sporulation, which takes about eight hours. The DNA is replicated and a membrane wall known as a spore septum begins to form between it and the rest of the cell. The plasma membrane of the cell surrounds this wall and pinches off to leave a double membrane around the DNA, and the developing structure is now known as a forespore. Calcium dipicolinate is incorporated into the forespore during this time. Next the peptidoglycan cortex forms between the two layers and the bacterium adds a spore coat to the outside of the forespore. Sporulation is now complete, and the mature endospore will be released when the surrounding vegetative cell is… [cont.]
Answered by Peter S - Wed Mar 25 22:40:30 2009
Q. help!! i can't find the definition anywhere on Google in an easy to understand way.
Asked by isabelle is cool - Wed Mar 25 22:27:38 2009 - - 1 Answers - 0 Comments
A. When a bacterium detects environmental conditions are becoming unfavourable it may start the process of sporulation, which takes about eight hours. The DNA is replicated and a membrane wall known as a spore septum begins to form between it and the rest of the cell. The plasma membrane of the cell surrounds this wall and pinches off to leave a double membrane around the DNA, and the developing structure is now known as a forespore. Calcium dipicolinate is incorporated into the forespore during this time. Next the peptidoglycan cortex forms between the two layers and the bacterium adds a spore coat to the outside of the forespore. Sporulation is now complete, and the mature endospore will be released when the surrounding vegetative cell is… [cont.]
Answered by Peter S - Wed Mar 25 22:40:30 2009
urgent! is Serratia marcescens a spore forming bacteria ?(endospore) ?
Q. cuz i read that Serratia marcescens is part of the bacullis family and most of the bacullis family are all spore forming bacteria. if yes can you give me a link for the references. thanks
Asked by dee - Mon Oct 13 21:26:38 2008 - - 1 Answers - 0 Comments
A. no, it is not. It is a Gram negative rod. As the name implies its genus and species is Serratia marcescens not Bacillus subtilus(for example).
Answered by barata.nikto - Mon Oct 13 22:43:42 2008
Q. cuz i read that Serratia marcescens is part of the bacullis family and most of the bacullis family are all spore forming bacteria. if yes can you give me a link for the references. thanks
Asked by dee - Mon Oct 13 21:26:38 2008 - - 1 Answers - 0 Comments
A. no, it is not. It is a Gram negative rod. As the name implies its genus and species is Serratia marcescens not Bacillus subtilus(for example).
Answered by barata.nikto - Mon Oct 13 22:43:42 2008
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Before this they are put into a sort of endospore like coma with a small amount of antibiotic called chloramphenicol. The ingredients for the moth spray can be found in the patents, however each patent is connected to another patent. ...
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An endospore stain of Bacillus subtilis using malachite green
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endospore
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