In gram stain, what would happen if failure to add iodine, fail to apply decolorizer, fail to apply safranin &?
Q. reversal of crystal violet and safranin stains?
Asked by Nancy * - Wed Jun 24 16:20:01 2009 - - 1 Answers - 0 Comments
A. Gram's iodine is a mordant. It chemically bonds the crystal violet to peptidoglycan. If you omit it, the Gram-positive cells will decolorize. If you forget to decolorize, everything will have crystal violet on it. If you don't counterstain with safranin, It will be difficult to visualize and identify Gram-negative organisms, and their thin cell walls may have bound enough crystal violet that they will be misidentified as Gram-positive.
Answered by novangelis - Wed Jun 24 17:45:45 2009
Q. reversal of crystal violet and safranin stains?
Asked by Nancy * - Wed Jun 24 16:20:01 2009 - - 1 Answers - 0 Comments
A. Gram's iodine is a mordant. It chemically bonds the crystal violet to peptidoglycan. If you omit it, the Gram-positive cells will decolorize. If you forget to decolorize, everything will have crystal violet on it. If you don't counterstain with safranin, It will be difficult to visualize and identify Gram-negative organisms, and their thin cell walls may have bound enough crystal violet that they will be misidentified as Gram-positive.
Answered by novangelis - Wed Jun 24 17:45:45 2009
What's the difference in dyes when staining endospores?
Q. What would happen if you used safranin as the primary stain and malachite green as the counter stain when dying a spore, instead of the other way around?
Asked by Ryan - Wed Jul 1 19:55:07 2009 - - 1 Answers - 0 Comments
A. If the heat can drive safranin into the endospore just like it does malachite green, then the spores would be pink and the rest of the cells would be green.
Answered by TheRunningMan - Thu Jul 2 01:48:31 2009
Q. What would happen if you used safranin as the primary stain and malachite green as the counter stain when dying a spore, instead of the other way around?
Asked by Ryan - Wed Jul 1 19:55:07 2009 - - 1 Answers - 0 Comments
A. If the heat can drive safranin into the endospore just like it does malachite green, then the spores would be pink and the rest of the cells would be green.
Answered by TheRunningMan - Thu Jul 2 01:48:31 2009
gram staining?
Q. what would happen if i chose to use safranin and then crystal violet?
Asked by Citi B - Wed Sep 19 17:04:09 2007 - - 1 Answers - 0 Comments
A. i believe the safranin stains everything...you just don't see it in the positive cells because they are already dark purple. My best guess is that the membrane would be saturated and the CV-I would not be able to stain. I suggest just doing it the correct way.
Answered by taylor p - Wed Sep 19 17:48:48 2007
Q. what would happen if i chose to use safranin and then crystal violet?
Asked by Citi B - Wed Sep 19 17:04:09 2007 - - 1 Answers - 0 Comments
A. i believe the safranin stains everything...you just don't see it in the positive cells because they are already dark purple. My best guess is that the membrane would be saturated and the CV-I would not be able to stain. I suggest just doing it the correct way.
Answered by taylor p - Wed Sep 19 17:48:48 2007
on Gram stain?
Q. what would happen if there was a mistake when performing a gram stain and there was a reversal of crystal violet and safranin stains?
Asked by sinajina - Thu Jun 26 08:26:24 2008 - - 1 Answers - 0 Comments
A. You would indeed get some blue bacteria under the microscope and no pink ones, a false result if there were indeed gram negative bacteria. One of the steps in Gram's method involves washing with ethanol/acetone to remove the crystal violet from gram negative bacteria, however if the steps were reversed the safranin would be washed out of both negatives and positives and the crystal violet would stick in both cell types. Bottom line? it would not work. Google is your friend.
Answered by Brooke B - Thu Jun 26 09:17:34 2008
Q. what would happen if there was a mistake when performing a gram stain and there was a reversal of crystal violet and safranin stains?
Asked by sinajina - Thu Jun 26 08:26:24 2008 - - 1 Answers - 0 Comments
A. You would indeed get some blue bacteria under the microscope and no pink ones, a false result if there were indeed gram negative bacteria. One of the steps in Gram's method involves washing with ethanol/acetone to remove the crystal violet from gram negative bacteria, however if the steps were reversed the safranin would be washed out of both negatives and positives and the crystal violet would stick in both cell types. Bottom line? it would not work. Google is your friend.
Answered by Brooke B - Thu Jun 26 09:17:34 2008
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